FastQCFastQC Report
Thu 11 May 2023
HH35LBGXT_n02_mab-3_dmd-3_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH35LBGXT_n02_mab-3_dmd-3_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45113947
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5907181.3093910847570043No Hit
CGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC3029450.6715107414565168No Hit
GCGCGCTGCATACTTGGATCGTCGGACTGTAGAACT1566580.34724959888790047No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT615380.13640571063312196Illumina RNA PCR Primer (100% over 31bp)
CGCTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA598980.13277047118045335No Hit
CGCGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC587000.1301149730924674No Hit
CCGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA531040.11771082676494699No Hit
CGTGCATGAGCGCGCTGCATACTTGGATCGTCGGAC501820.11123389403281429No Hit
CGTGTTTGAGCGCGCTGCATACTTGGATCGTCGGAC489420.10848529834908925No Hit
CGTTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA485910.1077072684418413No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)