FastQCFastQC Report
Thu 11 May 2023
HH35LBGXT_n02_mab-3_dmd-3_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH35LBGXT_n02_mab-3_dmd-3_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21491579
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2489261.1582490053429766No Hit
CGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC843120.392302492059797No Hit
GCGCGCTGCATACTTGGATCGTCGGACTGTAGAACT660520.30733898146804384No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT338300.15741049087179682Illumina RNA PCR Primer (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)