FastQCFastQC Report
Thu 11 May 2023
HH35LBGXT_n02_mab-3_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH35LBGXT_n02_mab-3_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45646572
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3485810.76365208760912No Hit
CGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC2506910.5492000582212395No Hit
CGTGTTTGAGCGCGCTGCATACTTGGATCGTCGGAC1839460.40297878228402345No Hit
CGTTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA1619160.3547166696329354No Hit
CGCTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA1316320.28837214763903846No Hit
GCGCGCTGCATACTTGGATCGTCGGACTGTAGAACT1240650.2717947801206189No Hit
TGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC1161650.2544878945126482No Hit
CGTGGCTTGAGCGCGCTGCATACTTGGATCGTCGGA1104130.24188672919403456No Hit
CCGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA1067440.23384888573888965No Hit
CGCGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC1052020.23047075692781485No Hit
CGTGCATGAGCGCGCTGCATACTTGGATCGTCGGAC1032990.22630176916680622No Hit
CGTCGCTTGAGCGCGCTGCATACTTGGATCGTCGGA886360.19417887503140435No Hit
TGTGTTTGAGCGCGCTGCATACTTGGATCGTCGGAC862000.1888422201781111No Hit
CTGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA839860.18399191071785193No Hit
CGATGCTTGAGCGCGCTGCATACTTGGATCGTCGGA797880.1747951631504771No Hit
TCGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA780630.17101612800190122No Hit
ACGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA771670.16905322046965543No Hit
CGTAGCTTGAGCGCGCTGCATACTTGGATCGTCGGA760560.16661930275947118No Hit
TGTTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA753040.16497186250919346No Hit
CAGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA631690.1383871717683422No Hit
CGTGCTTGAGCGCGCTGCATACTTGATCGTCGGACT581150.12731514646926828No Hit
TTGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA529770.1160590985890463No Hit
CGTGTCTTGAGCGCGCTGCATACTTGGATCGTCGGA517630.11339953414245434No Hit
CGTGACTTGAGCGCGCTGCATACTTGGATCGTCGGA515220.11287156459416055No Hit
CGCCGCTTGAGCGCGCTGCATACTTGGATCGTCGGA513040.11239398218118109No Hit
CGTGATTGAGCGCGCTGCATACTTGGATCGTCGGAC506860.11104010176273477No Hit
CGGCGCTTGAGCGCGCTGCATACTTGGATCGTCGGA484660.10617664783239363No Hit
CGCTGCATACTTGGATCGTCGGACTGTAGAACTCTG477610.10463217259775827Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGCTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA468380.10261011495014347No Hit
CGTGTTTGAGCGCGCTGCATACTTGATCGTCGGACT457040.10012581010464489No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)