FastQCFastQC Report
Thu 11 May 2023
HH35LBGXT_n02_mab-3_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH35LBGXT_n02_mab-3_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43098451
Sequences flagged as poor quality0
Sequence length36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5839991.3550347783960959No Hit
CGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC1696220.3935686690920748No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT1119810.25982604340002846Illumina RNA PCR Primer (100% over 31bp)
GCGCGCTGCATACTTGGATCGTCGGACTGTAGAACT1044570.24236833940969246No Hit
CGCTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA485470.1126420993645456No Hit
CGCGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC476040.1104540856932422No Hit
CCGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA444220.10307098972072105No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)