FastQCFastQC Report
Thu 11 May 2023
HH35LBGXT_n02_mab-3_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH35LBGXT_n02_mab-3_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27456069
Sequences flagged as poor quality0
Sequence length36
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2012840.7331129594699082No Hit
CGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC2011760.7327196038150983No Hit
CGTGTTTGAGCGCGCTGCATACTTGGATCGTCGGAC1366460.49768960006620033No Hit
CGTTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA1299260.4732141371002528No Hit
CGCTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA1255410.4572431690785742No Hit
CGCGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC1014810.3696122704237085No Hit
TGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC878400.3199292659120284No Hit
CGTGGCTTGAGCGCGCTGCATACTTGGATCGTCGGA850380.30972387197890566No Hit
CGTGCATGAGCGCGCTGCATACTTGGATCGTCGGAC839720.30584130597865267No Hit
CGTCGCTTGAGCGCGCTGCATACTTGGATCGTCGGA808070.2943137999835301No Hit
CCGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA805100.2932320719328029No Hit
GCGCGCTGCATACTTGGATCGTCGGACTGTAGAACT796570.29012529069620274No Hit
CTGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA621200.22625234515545545No Hit
CGATGCTTGAGCGCGCTGCATACTTGGATCGTCGGA617090.22475540835798455No Hit
TGTGTTTGAGCGCGCTGCATACTTGGATCGTCGGAC609700.22206383586812808No Hit
TCGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA597380.2175766676577044No Hit
ACGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA587150.21385071548297757No Hit
CGTAGCTTGAGCGCGCTGCATACTTGGATCGTCGGA573920.20903210871155664No Hit
TGTTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA550310.20043291703557417No Hit
CGCCGCTTGAGCGCGCTGCATACTTGGATCGTCGGA495180.18035356773032585No Hit
CGGCGCTTGAGCGCGCTGCATACTTGGATCGTCGGA464050.16901545519863023No Hit
CAGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA448740.1634392745735014No Hit
TGCTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA398690.14521015371865506No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT393430.14329436599245143No Hit
CGTGTCTTGAGCGCGCTGCATACTTGGATCGTCGGA392500.14295564306747627No Hit
CGCGTTTGAGCGCGCTGCATACTTGGATCGTCGGAC391940.14275168087609336No Hit
TTGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA388170.1413785782662478No Hit
CGTGACTTGAGCGCGCTGCATACTTGGATCGTCGGA385880.14054451859077133No Hit
CGTGCTTGAGCGCGCTGCATACTTGATCGTCGGACT372480.13566399472553775No Hit
GCGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA354770.12921369042305364No Hit
CGTGATTGAGCGCGCTGCATACTTGGATCGTCGGAC349890.12743630561243127No Hit
CTGCGCTTGAGCGCGCTGCATACTTGGATCGTCGGA342880.1248831360381561No Hit
CGCTGCATACTTGGATCGTCGGACTGTAGAACTCTG342540.12475930185053075Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTGCTTGAGCGCGCTGCATACTTGGATCGTCGGACT336010.12238095701172663No Hit
CATGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC328600.11968210015789224No Hit
TGCAGAATTGTTTAAACGGTTAATTAAGGCTAGAAA320410.11669915310891738No Hit
CGTGCATTGAGCGCGCTGCATACTTGGATCGTCGGA319740.11645512691565571No Hit
TGTGGCTTGAGCGCGCTGCATACTTGGATCGTCGGA307220.11189511506545238No Hit
GCGCTGCATACTTGGATCGTCGGACTGTAGAACTCT304260.11081702919671423Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCGTGTTTGAGCGCGCTGCATACTTGGATCGTCGGA304000.11072233246500073No Hit
CGAGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC298400.10868271055117176No Hit
GCTGCATACTTGGATCGTCGGACTGTAGAACTCTGA297040.10818737380067045Illumina DpnII expression Sequencing Primer (100% over 24bp)
TGTTTTTTTGATCGTCGGACTGTAGAACTCTGAACG296160.10786686178564017Illumina DpnII expression Sequencing Primer (96% over 26bp)
CAGCGCTTGAGCGCGCTGCATACTTGGATCGTCGGA294960.10742979994696254No Hit
CGTGCGTGAGCGCGCTGCATACTTGGATCGTCGGAC290240.10571069004816384No Hit
CGGCTTGAGCGCGCTGCATACTTGGATCGTCGGACT290040.10563784640838425No Hit
CCGTGTTTGAGCGCGCTGCATACTTGGATCGTCGGA289760.1055358653126928No Hit
CGACGCTTGAGCGCGCTGCATACTTGGATCGTCGGA288270.10499318019633473No Hit
TAGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGA281940.10268767899731021No Hit
CGTTGTTTGAGCGCGCTGCATACTTGGATCGTCGGA280560.10218505788283094No Hit
CTGTGTTTGAGCGCGCTGCATACTTGGATCGTCGGA275360.1002911232485612No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)