FastQCFastQC Report
Thu 11 May 2023
HH35LBGXT_n02_dmd-3_28_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH35LBGXT_n02_dmd-3_28_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39836463
Sequences flagged as poor quality0
Sequence length36
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5424511.3616946865990587No Hit
CGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC1416740.35563900339244475No Hit
GCGCGCTGCATACTTGGATCGTCGGACTGTAGAACT1152990.28943081618465977No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT632520.15877915667362336Illumina RNA PCR Primer (100% over 31bp)
TGTTTTTTTGATCGTCGGACTGTAGAACTCTGAACG405590.10181375791319626Illumina DpnII expression Sequencing Primer (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)