FastQCFastQC Report
Thu 11 May 2023
HH35LBGXT_n01_mab-3_dmd-3_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH35LBGXT_n01_mab-3_dmd-3_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48910119
Sequences flagged as poor quality0
Sequence length15
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTATGCAGCGCG19788144.045817185601205No Hit
AAAAAAAAAAAAAAA10108352.0667195677851447No Hit
CAAAAAAAAAAAAAA7348051.5023578249727834No Hit
AAGTATGCAGCGCGC3093120.6324090113131804No Hit
CCAAAAAAAAAAAAA2137150.43695456966686175No Hit
CCAAGTATGCAGCGC1389910.28417636849339906No Hit
TAAAAAAAAAAAAAA1379590.28206637567166826No Hit
GAAAAAAAAAAAAAA1112390.2274355537756921No Hit
TGGAATTCTCGGGTG917990.1876891773663442Illumina Small RNA Adapter 2 (100% over 15bp)
TGCAACTCCAGTCTT839680.1716781756347802No Hit
ACAAAAAAAAAAAAA614440.1256263555604925No Hit
AAAGTATGCAGCGCG609550.12462656245019563No Hit
AAAAAAAACATGGTG555960.11366972957068454No Hit
CCCAAAAAAAAAAAA515810.10546079431947405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)