FastQCFastQC Report
Thu 11 May 2023
HH35LBGXT_n01_mab-3_dmd-3_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH35LBGXT_n01_mab-3_dmd-3_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27013279
Sequences flagged as poor quality0
Sequence length15
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTATGCAGCGCG5783482.140976665587321No Hit
AAAAAAAAAAAAAAA3918591.450616195094272No Hit
CAAAAAAAAAAAAAA3254311.2047075070005384No Hit
AAGTATGCAGCGCGC998200.36952196732577336No Hit
CCAAAAAAAAAAAAA880920.32610628276559833No Hit
TAAAAAAAAAAAAAA575110.21289899682300695No Hit
GAAAAAAAAAAAAAA519120.19217215355455367No Hit
CCAAGTATGCAGCGC454400.16821356637230156No Hit
TGGAATTCTCGGGTG407910.15100351201348047Illumina Small RNA Adapter 2 (100% over 15bp)
AAAGTATGCAGCGCG309700.11464731845400923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)