FastQCFastQC Report
Thu 11 May 2023
HH35LBGXT_n01_mab-3_dmd-3_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH35LBGXT_n01_mab-3_dmd-3_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45113947
Sequences flagged as poor quality0
Sequence length15
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTATGCAGCGCG23694425.252127462932915No Hit
AAAAAAAAAAAAAAA7558961.6755261959234027No Hit
CAAAAAAAAAAAAAA7192941.594393857846222No Hit
AAGTATGCAGCGCGC2544640.564047300051135No Hit
CCAAAAAAAAAAAAA2133400.4728914541660476No Hit
TAAAAAAAAAAAAAA1439060.31898339553397975No Hit
CCAAGTATGCAGCGC1212220.2687018273971905No Hit
GAAAAAAAAAAAAAA1131700.25085368832835664No Hit
TGGAATTCTCGGGTG739890.1640047145509126Illumina Small RNA Adapter 2 (100% over 15bp)
ACAAAAAAAAAAAAA575420.12754813938137577No Hit
CCCAAAAAAAAAAAA490500.10872469216670401No Hit
TGCAACTCCAGTCTT488600.10830353637645582No Hit
CAAGTATGCAGCGCC473510.10495867275811624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)