FastQCFastQC Report
Thu 11 May 2023
HH35LBGXT_n01_mab-3_dmd-3_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH35LBGXT_n01_mab-3_dmd-3_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21491579
Sequences flagged as poor quality0
Sequence length15
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTATGCAGCGCG6538963.0425684404110096No Hit
AAAAAAAAAAAAAAA2054550.9559790837146028No Hit
CAAAAAAAAAAAAAA1762440.8200607316940276No Hit
AAGTATGCAGCGCGC765420.356148796698465No Hit
CCAAGTATGCAGCGC543570.25292231901620627No Hit
CCAAAAAAAAAAAAA490990.22845692259279787No Hit
TGGAATTCTCGGGTG404000.1879806039379424Illumina Small RNA Adapter 2 (100% over 15bp)
TAAAAAAAAAAAAAA334230.15551672587667942No Hit
GAAAAAAAAAAAAAA277070.12892026221060815No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)