FastQCFastQC Report
Thu 11 May 2023
HH35LBGXT_n01_mab-3_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH35LBGXT_n01_mab-3_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45646572
Sequences flagged as poor quality0
Sequence length15
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTATGCAGCGCG693467315.192100296162437No Hit
AAGTATGCAGCGCGC16999663.7241920379037445No Hit
AAAAAAAAAAAAAAA8373811.8344882502896385No Hit
CAAAAAAAAAAAAAA8224351.8017453753153687No Hit
CCAAGTATGCAGCGC4947061.083774702731237No Hit
AAAGTATGCAGCGCG4044160.8859723354472271No Hit
ACAGTATGCAGCGCG3374310.7392252807067308No Hit
ATAGTATGCAGCGCG3320150.7273602057127093No Hit
CCAAAAAAAAAAAAA2489610.5454100693475953No Hit
TAAGTATGCAGCGCG2370290.5192700998445184No Hit
CCAGTATGCAGCGCG2072750.45408667270786507No Hit
AGAGTATGCAGCGCG1650860.3616613313262604No Hit
TGCAACTCCAGTCTT1645970.36059005701457714No Hit
CTAGTATGCAGCGCG1555520.3407747683659575No Hit
TAAAAAAAAAAAAAA1259770.2759834845867506No Hit
TTAGTATGCAGCGCG1157660.25361378725219497No Hit
GAAAAAAAAAAAAAA1135090.24866927575634815No Hit
CAAGTATGCAGCCGC1111550.2435122619941756No Hit
GAAGTATGCAGCGCG1108960.24294485903563579No Hit
CAAGTATGCAGCGCC1071090.23464850766887818No Hit
CAAGTATGCAGCGCA823110.18032241281995942No Hit
AATGTATGCAGCGCG817360.17906273443710077No Hit
ACAAGTATGCAGCGC765420.16768400483611343No Hit
CAAGTATGCAGCGCT750110.16432997422018897No Hit
CAAGTATGCAGCGGC723200.1584346793884106No Hit
CGAGTATGCAGCGCG722360.15825065680726255No Hit
CCCAAAAAAAAAAAA658000.14415102189930057No Hit
GTAGTATGCAGCGCG638810.13994698221807325No Hit
CAAGTATGCAGCGTG601370.1317448328869033No Hit
TCAAGTATGCAGCGC598620.13114237800814485No Hit
ACAAAAAAAAAAAAA529640.11603061890386862No Hit
CAAGTATGCAGGCGC504120.11043983762898998No Hit
CAAGTATGCAGCTGG470410.1030548361879179No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)