FastQCFastQC Report
Thu 11 May 2023
HH35LBGXT_n01_mab-3_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH35LBGXT_n01_mab-3_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43098451
Sequences flagged as poor quality0
Sequence length15
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTATGCAGCGCG19711404.573575045655353No Hit
AAAAAAAAAAAAAAA5952731.381193491153545No Hit
CAAAAAAAAAAAAAA5584091.2956590945693152No Hit
AAGTATGCAGCGCGC3715790.8621632364467113No Hit
CCAAAAAAAAAAAAA1732170.40191003616348064No Hit
CCAAGTATGCAGCGC1574460.36531707369250926No Hit
TGGAATTCTCGGGTG1330470.30870483025016376Illumina Small RNA Adapter 2 (100% over 15bp)
AAAGTATGCAGCGCG1326660.30782080775942505No Hit
TAAAAAAAAAAAAAA1232790.28604044261358724No Hit
GAAAAAAAAAAAAAA917840.21296357031485888No Hit
ATAGTATGCAGCGCG664720.15423292127134683No Hit
ACAGTATGCAGCGCG654430.15184536446565097No Hit
TAAGTATGCAGCGCG617830.14335317990894847No Hit
ACAAAAAAAAAAAAA509210.11815041798137942No Hit
CCAGTATGCAGCGCG497840.11551227212319068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)