FastQCFastQC Report
Thu 11 May 2023
HH35LBGXT_n01_mab-3_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH35LBGXT_n01_mab-3_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27456069
Sequences flagged as poor quality0
Sequence length15
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTATGCAGCGCG549884120.02777965046635No Hit
AAGTATGCAGCGCGC10971443.9959981161177884No Hit
AAAAAAAAAAAAAAA6527272.377350523121136No Hit
CAAAAAAAAAAAAAA5471731.9929036454563107No Hit
CCAAGTATGCAGCGC3007361.0953352426379757No Hit
TGCAACTCCAGTCTT2706270.9856727851317683No Hit
AAAGTATGCAGCGCG2519160.9175239179359579No Hit
ATAGTATGCAGCGCG2132940.7768555651575614No Hit
ACAGTATGCAGCGCG1659610.6044601650731574No Hit
CCAAAAAAAAAAAAA1632660.5946444846128556No Hit
TAAGTATGCAGCGCG1225890.44649144784710443No Hit
CCAGTATGCAGCGCG1036070.3773555493322806No Hit
TAAAAAAAAAAAAAA923310.3362863052245389No Hit
CAAGTATGCAGCGCC879800.32043917139048567No Hit
AGAGTATGCAGCGCG875830.3189932251408605No Hit
CAAGTATGCAGCCGC840830.3062455881794295No Hit
GAAAAAAAAAAAAAA755170.2750466572618243No Hit
CTAGTATGCAGCGCG737270.26852715150154965No Hit
GAAGTATGCAGCGCG697930.25419880755690116No Hit
TTAGTATGCAGCGCG680630.24789783271596527No Hit
CAAGTATGCAGCGGC650600.23696036020305747No Hit
CAAGTATGCAGCGCA639090.23276820873374118No Hit
CAAGTATGCAGCGCT535930.19519545933542054No Hit
ACAAGTATGCAGCGC520580.18960470998233578No Hit
AATGTATGCAGCGCG461920.16823967043497742No Hit
CAAGTATGCAGCGTG442840.16129038720000302No Hit
GTAGTATGCAGCGCG405610.1477305436550294No Hit
CCCAAAAAAAAAAAA379120.13808240356622065No Hit
CAAGTATGCAGGCGC376630.13717550025096456No Hit
TCAAGTATGCAGCGC367680.1339157473708272No Hit
CAAGTATGCAGCTGG355100.12933388242869No Hit
AAAAAAACATGGAAT352420.12835777765564327No Hit
ACAAAAAAAAAAAAA298620.10876283855492933No Hit
CGAGTATGCAGCGCG278890.10157681349067123No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)