FastQCFastQC Report
Thu 11 May 2023
HH35LBGXT_n01_mab-3_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH35LBGXT_n01_mab-3_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42414153
Sequences flagged as poor quality0
Sequence length15
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAA8030051.893247756238348No Hit
CAAGTATGCAGCGCG5750691.3558422350199943No Hit
CAAAAAAAAAAAAAA5508581.2987598738562574No Hit
AAGTATGCAGCGCGC1629840.3842679588579784No Hit
CCAAAAAAAAAAAAA1455910.3432604206430811No Hit
TGGAATTCTCGGGTG1092350.25754374960641085Illumina Small RNA Adapter 2 (100% over 15bp)
TAAAAAAAAAAAAAA1046540.24674310954647616No Hit
GAAAAAAAAAAAAAA802370.1891750614470599No Hit
AAAGTATGCAGCGCG697460.16444039328098808No Hit
ACAGTATGCAGCGCG500970.11811387580933186No Hit
ACAAAAAAAAAAAAA494760.11664974189158038No Hit
AAAAAAAACATGGTG458240.10803940844934473No Hit
AAAAAAACATGGTGG436540.10292319169971402No Hit
TAAGTATGCAGCGCG435970.10278880259615229No Hit
TTTTTTCTGTGATTT425730.10037451413918368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)