FastQCFastQC Report
Thu 11 May 2023
HH35LBGXT_n01_dmd-3_28_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH35LBGXT_n01_dmd-3_28_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39836463
Sequences flagged as poor quality0
Sequence length15
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAA12727793.1950100590004693No Hit
CAAGTATGCAGCGCG10690072.6834887424618996No Hit
CAAAAAAAAAAAAAA8994562.2578711368024815No Hit
AAGTATGCAGCGCGC2995080.7518438572219627No Hit
CCAAAAAAAAAAAAA2203420.5531163748147018No Hit
TAAAAAAAAAAAAAA1407350.3532818664146965No Hit
GAAAAAAAAAAAAAA1278900.3210375378958719No Hit
AAAGTATGCAGCGCG907340.22776620504686876No Hit
CCAAGTATGCAGCGC831750.20879112686284423No Hit
ACAAAAAAAAAAAAA820460.2059570399108977No Hit
TGGAATTCTCGGGTG746240.18732586776090038Illumina Small RNA Adapter 2 (100% over 15bp)
AAAAAAAACATGGTG593180.14890378194469725No Hit
ACAGTATGCAGCGCG590410.14820843908757664No Hit
ATAGTATGCAGCGCG510590.12817151964520543No Hit
AAAAAAACATGGAAT481110.1207712642560661No Hit
GCAAAAAAAAAAAAA453580.11386051015623551No Hit
CCAGTATGCAGCGCG435650.10935960855761719No Hit
TAAGTATGCAGCGCG431370.10828521598416005No Hit
TCAAAAAAAAAAAAA427010.10719074130652614No Hit
AAAAAAACATGGTGG426420.10704263578822247No Hit
ACCAAAAAAAAAAAA403570.10130668478273284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)