FastQCFastQC Report
Thu 11 May 2023
HH35LBGXT_n01_WT_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH35LBGXT_n01_WT_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29000166
Sequences flagged as poor quality0
Sequence length15
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAA2609310.8997569186328106No Hit
CAAAAAAAAAAAAAA2013770.6943994734374969No Hit
CAAGTATGCAGCGCG1434640.4947006165412984No Hit
TGGAATTCTCGGGTG1086720.37472888948290844Illumina Small RNA Adapter 2 (100% over 15bp)
CCAAAAAAAAAAAAA464280.16009563531463922No Hit
TAAAAAAAAAAAAAA414810.14303711227032287No Hit
CCCTACAGACACATT389350.13425785217919098No Hit
GAAAAAAAAAAAAAA342840.1182200129475121No Hit
TGCAACTCCAGTCTT315840.1089097214133188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)