FastQCFastQC Report
Sat 18 Aug 2018
HH2YKBGX7_n01_1brs.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2YKBGX7_n01_1brs.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48.695652173913043No Hit
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24.3478260869565215No Hit
CGGGGGAGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.1739130434782608No Hit
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCAAAAACC12.1739130434782608No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACACC12.1739130434782608No Hit
GGCGGAAAAAAAAAAAACAAAAAAAAAAAAAGAGAACGGCAGAGCACCAG12.1739130434782608No Hit
GGGGGGAGAAAAAAAAAAAAAAAAGAGGGGAAGGGCACCCGTGTGAACAC12.1739130434782608No Hit
GGGGGGAGAAAAAAAAAAAAAAGAAGGGAAGGGGCCACGTGGGAAAACCA12.1739130434782608No Hit
CCGGGCGAAAAAAAAAAAAAAAGATCGGCGGAGCACCCCACTGAACACCA12.1739130434782608No Hit
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.1739130434782608No Hit
CCGGGGGGGGAAAAAAAAAAAAAACGGAAGGGAACACGACGGTAGTCACT12.1739130434782608No Hit
GGGAGAAAAAAAAAAGAAAAAAAAAAAAGAAGAAGGCAACGGCACACGTC12.1739130434782608No Hit
CGGGGGATAAAAAAAAAAAAGATCGGAGGAGCACACCACTGAACTCCAAA12.1739130434782608No Hit
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAA12.1739130434782608No Hit
CGGGGAAAAAAAAAAGAAAAAGAAAAGAAAAGCACGCGTCTGCACACCTA12.1739130434782608No Hit
CTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC12.1739130434782608No Hit
CGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAACAAA12.1739130434782608No Hit
CGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAA12.1739130434782608No Hit
CCGGGGAGAAAAGAAAAAAAAAAGAACGGAGCGGAACCGGCCTCAACTCC12.1739130434782608No Hit
CGGGGAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAACCAA12.1739130434782608No Hit
CGGGGAAAAAAAAAGAAAAAAAAAAAAAAAGAAAGGAAGGGCACACGTAT12.1739130434782608No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCACAA12.1739130434782608No Hit
CGGGGAAAAAAAAAAAAAAAAAAAAAAACAAAAGAAAACACCTCTAAACC12.1739130434782608No Hit
AGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.1739130434782608No Hit
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGCCGCGCACGAGTAA12.1739130434782608No Hit
CGGGAAGAAAAAAAAAAAAAAAAAGAGGGGGAGCCCACCACTGAACACCA12.1739130434782608No Hit
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.1739130434782608No Hit
GGGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12.1739130434782608No Hit
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAGGAAGCGCACCCGG12.1739130434782608No Hit
CGGGGGAGAAAAAAAAAAAAAAAAAAGAAAAAAAAAACGGAACAAAACCA12.1739130434782608No Hit
CGGGAAAAAAAAAAAAAAAAAAAAAAACGGAAGAGACCCCGGCGAACAAC12.1739130434782608No Hit
CGGGGGAGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCC12.1739130434782608Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA12.1739130434782608No Hit
CCGGAAAAGAAAAAAAAAAAAAAAAATAGGAAGAGCACACGTCTGAACTC12.1739130434782608Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAACAAAC12.1739130434782608No Hit
GGGGGGAGAGAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTC12.1739130434782608Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGGGAAAAAAAAAAAAAAGATCGGAGGAGCACACGACTGAACACACCT12.1739130434782608No Hit
CGGGAAAAAAAAAAAAAAAAAAAAAAGAGCGGAAGAGCACACGTCTCACC12.1739130434782608Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAACAA12.1739130434782608No Hit
GCGGGAGGAACTAAAAAAAAAAGAAAGGAAGGGCACACGACAGCACTACA12.1739130434782608No Hit
GGGGGGAGAAAAAAAAAAAAAAGAAGCAGAAAACAAAAAAAAAAAAACCC12.1739130434782608No Hit
CGGGAAAAAAAAAAAAAAAAAAAAAAGAAAGAGCAAAAGAAAAAACAACA12.1739130434782608No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)