FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_5000_250_2_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_5000_250_2_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13440386
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC318190.23674171262640817TruSeq Adapter, Index 4 (100% over 50bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC141320.1051457897116943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG52500.052.06414846
CGTATGC56500.049.0593944
TCGTATG58600.047.65963443
CTCGTAT53950.047.2268142
GTATGCC58500.047.0235445
ACTGACC62350.044.8498132
AGTCACT63650.044.20970528
ACGTCTG66350.042.83202415
CAGTCAC66950.041.92571627
CACGTCT68500.041.74297314
ACACGTC73450.038.78655613
ATGCCGT73800.037.27475747
GTCTGAA78950.036.12858217
CACACGT82650.034.59615712
CTGACCA83000.034.23943733
TCTCGTA75500.033.42222641
CCAGTCA84450.033.40363726
CCGTCTT82600.033.0504850
GTCACTG85750.033.01979429
ACCAATC76250.032.95602836
Produced by FastQC (version 0.11.2)