FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_5000_250_1_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_5000_250_1_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10964606
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGC621050.5664134215128205TruSeq Adapter, Index 3 (97% over 36bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC142900.13032844043826108No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA132200.12056976785121143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG81900.060.38151646
CTCGTAT76950.059.26271442
CGTATGC84300.059.2024544
TCGTATG83450.058.92474743
GTATGCC85100.058.23437545
AGTCACT91550.055.89085428
GTCACTC91600.055.70750829
ACGTCTG92850.055.07238415
CAGTCAC94550.054.33957727
CACGTCT94550.054.1932414
ATGCCGT91400.054.06798647
TCACTCA96550.052.92298530
ACACGTC97750.052.70582213
GTCTGAA100450.051.18181217
CACTCAT100450.050.69426331
CCGTCTT98750.049.7617450
GCCGTCT99650.049.2420649
AGCACAC109400.047.25357410
TGCCGTC105050.046.8769848
CTGAACT111250.046.30752619
Produced by FastQC (version 0.11.2)