FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_5000_125_2_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_5000_125_2_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15083103
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTCGTATGC252890.16766443880944126TruSeq Adapter, Index 6 (97% over 36bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC243520.16145218924779603No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA190240.12612789291434262No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA175820.11656752592619701No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC168100.11144921572172517No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA167970.11136302656025089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG38700.050.37430228
TATGCCG38650.048.98879246
CGTATGC41000.046.69260444
TCGTATG41750.045.85366443
CTCGTAT41100.045.04608542
GTCACGA44800.044.1404429
ACGTCTG45200.043.5943715
GTATGCC44700.042.98468845
CACGTCT47600.041.39647314
CAGTCAC50600.038.80405427
ACACGTC53150.037.40304613
GTCTGAA56600.035.18338417
ATGCCGT57600.033.35824247
ACGACAT61800.030.97729535
AGCACAC67700.030.29559710
CACACGT68950.028.83217612
GCCGTCT67300.028.65468249
CCAGTCA68650.028.4995226
CTGAACT70800.028.42351519
CCGTCTT68450.027.81552950
Produced by FastQC (version 0.11.2)