FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_5000_125_1_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_5000_125_1_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16791477
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC322260.1919187930877075TruSeq Adapter, Index 2 (97% over 36bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC245370.1461277051446993No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA218290.13000047583664023No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC203910.12143660739314356No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA196760.11717849478041747No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA188350.1121700014834907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG53650.051.66539846
CTCGTAT52150.050.3331242
CGTATGC56400.049.5187244
TCGTATG56950.049.40921443
GTATGCC58950.047.25808745
AGTCACC63200.044.30230328
CAGTCAC64400.043.80286827
CACGTCT65650.043.609114
ACGTCTG66400.043.27465415
ACACGTC70900.040.37993613
GTCTGAA72350.039.520417
ATGCCGT71900.038.50299547
CACCCAA76850.036.5231631
CCGTCTT79750.034.23163650
AGCACAC86100.033.78045310
GCCGTCT81700.033.4571449
CCAGTCA84500.033.21801426
CTGAACT88250.032.55893319
ACCCAAC87800.032.16823632
CACACGT91050.031.59714312
Produced by FastQC (version 0.11.2)