FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_5000_062_2_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_5000_062_2_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13316959
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC206210.1548476645456369TruSeq Adapter, Index 7 (97% over 35bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC175180.1315465490281978No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC140390.10542196608099492No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA138520.10401774158800069No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA136120.10221552833495995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG30900.051.9883246
TCGTATG32750.049.37143343
CTCGTAT30000.048.99740642
GTATGCC34250.047.31189745
CGTATGC36000.045.30332644
ACGTCTG39050.043.558715
CACGTCT40200.042.3126214
CAGTCAC39600.042.15796727
ACTCAAT38550.041.94424435
AGTCACC39900.041.4901228
ATGCCGT40900.039.7056947
ACACGTC42900.039.56785613
CCACTCA42500.038.375333
GTCTGAA45200.037.86289617
CTCAATC40400.036.298436
GCCGTCT48300.033.62304749
AGCACAC50750.033.30995610
CCGTCTT50050.032.37748750
CTGAACT53100.032.2960819
CACCACT52850.031.25697531