Basic Statistics
Measure | Value |
---|---|
Filename | HH2TCBGX7_n01_5000_062_2_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 13316959 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC | 20621 | 0.1548476645456369 | TruSeq Adapter, Index 7 (97% over 35bp) |
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC | 17518 | 0.1315465490281978 | No Hit |
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC | 14039 | 0.10542196608099492 | No Hit |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA | 13852 | 0.10401774158800069 | No Hit |
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA | 13612 | 0.10221552833495995 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 3090 | 0.0 | 51.98832 | 46 |
TCGTATG | 3275 | 0.0 | 49.371433 | 43 |
CTCGTAT | 3000 | 0.0 | 48.997406 | 42 |
GTATGCC | 3425 | 0.0 | 47.311897 | 45 |
CGTATGC | 3600 | 0.0 | 45.303326 | 44 |
ACGTCTG | 3905 | 0.0 | 43.5587 | 15 |
CACGTCT | 4020 | 0.0 | 42.31262 | 14 |
CAGTCAC | 3960 | 0.0 | 42.157967 | 27 |
ACTCAAT | 3855 | 0.0 | 41.944244 | 35 |
AGTCACC | 3990 | 0.0 | 41.49012 | 28 |
ATGCCGT | 4090 | 0.0 | 39.70569 | 47 |
ACACGTC | 4290 | 0.0 | 39.567856 | 13 |
CCACTCA | 4250 | 0.0 | 38.3753 | 33 |
GTCTGAA | 4520 | 0.0 | 37.862896 | 17 |
CTCAATC | 4040 | 0.0 | 36.2984 | 36 |
GCCGTCT | 4830 | 0.0 | 33.623047 | 49 |
AGCACAC | 5075 | 0.0 | 33.309956 | 10 |
CCGTCTT | 5005 | 0.0 | 32.377487 | 50 |
CTGAACT | 5310 | 0.0 | 32.29608 | 19 |
CACCACT | 5285 | 0.0 | 31.256975 | 31 |