FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_5000_062_1_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_5000_062_1_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7889146
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT245900.3116940667595707TruSeq Adapter, Index 27 (97% over 44bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC115750.14672057026197766No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC95040.12046931315506139No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA90810.11510751607334939No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA89970.11404276204293848No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA84460.10705848262917178No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA81400.1031797358041035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT29350.060.21870444
TATGCCG30850.059.901948
TCGTATG31400.058.51716645
GTATGCC32500.056.5369447
AGTCACA33950.055.4627828
CGTATGC33900.054.61470446
TTATCTC32400.054.3335840
ACATTCC34500.053.9688832
CAGTCAC35200.053.3937927
GTCACAT35250.053.21842229
ATGCCGT34850.052.9260849
ACGTCTG35950.052.86400215
CACGTCT36600.052.2117114
ACACGTC37950.050.99994713
GTCTGAA37900.050.14281517
CACATTC37450.049.71734231
GCCGTCT37600.048.3107951
CTGAACT40100.047.6536719
TGCCGTC39950.045.99463350
CCGTCTT39500.045.98668752