Basic Statistics
Measure | Value |
---|---|
Filename | HH2TCBGX7_n01_5000_062_1_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 7889146 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT | 24590 | 0.3116940667595707 | TruSeq Adapter, Index 27 (97% over 44bp) |
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC | 11575 | 0.14672057026197766 | No Hit |
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC | 9504 | 0.12046931315506139 | No Hit |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA | 9081 | 0.11510751607334939 | No Hit |
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA | 8997 | 0.11404276204293848 | No Hit |
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA | 8446 | 0.10705848262917178 | No Hit |
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA | 8140 | 0.1031797358041035 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGTAT | 2935 | 0.0 | 60.218704 | 44 |
TATGCCG | 3085 | 0.0 | 59.9019 | 48 |
TCGTATG | 3140 | 0.0 | 58.517166 | 45 |
GTATGCC | 3250 | 0.0 | 56.53694 | 47 |
AGTCACA | 3395 | 0.0 | 55.46278 | 28 |
CGTATGC | 3390 | 0.0 | 54.614704 | 46 |
TTATCTC | 3240 | 0.0 | 54.33358 | 40 |
ACATTCC | 3450 | 0.0 | 53.96888 | 32 |
CAGTCAC | 3520 | 0.0 | 53.39379 | 27 |
GTCACAT | 3525 | 0.0 | 53.218422 | 29 |
ATGCCGT | 3485 | 0.0 | 52.92608 | 49 |
ACGTCTG | 3595 | 0.0 | 52.864002 | 15 |
CACGTCT | 3660 | 0.0 | 52.21171 | 14 |
ACACGTC | 3795 | 0.0 | 50.999947 | 13 |
GTCTGAA | 3790 | 0.0 | 50.142815 | 17 |
CACATTC | 3745 | 0.0 | 49.717342 | 31 |
GCCGTCT | 3760 | 0.0 | 48.31079 | 51 |
CTGAACT | 4010 | 0.0 | 47.65367 | 19 |
TGCCGTC | 3995 | 0.0 | 45.994633 | 50 |
CCGTCTT | 3950 | 0.0 | 45.986687 | 52 |