FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_5000_031_2_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_5000_031_2_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14722947
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT147300.1000479048114484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG28850.043.1879828
TATGCCG29750.040.58667448
CTCGTAT29400.040.11695544
TCGTATG32900.037.23197645
CGTATGC33100.036.5840746
GTCACGA35050.035.74779529
GTATGCC33950.035.56491547
ACGTCTG38900.033.73963515
TCACGAG38200.032.89084230
CACGTCT40150.032.6894314
ACGAGTG39250.031.83327932
CAGTCAC39700.031.73724627
CACGAGT40500.030.93676631
ACACGTC43750.030.0797613
GTCTGAA46900.028.13247917
GTACTCT48100.026.0522543
ATGCCGT47950.025.6925349
TTACCTC47700.025.24054515
TGTTACC49350.024.8934213
TCTAGTT53350.024.0100777
Produced by FastQC (version 0.11.2)