FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_5000_031_1_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_5000_031_1_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8243937
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT423030.5131407481643783TruSeq Adapter, Index 19 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG316950.38446436453844807No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG196630.23851468054644281No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC98180.1190935835632902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG60850.058.1498948
CGTATGC62050.056.96787646
TCGTATG63100.055.90830645
GTATGCC63950.055.27532247
CTCGTAT59450.054.86674544
GAAACGA68200.051.2668736
TGAAACG70250.050.91639335
ATGCCGT71550.049.405849
AGTCACG73550.048.3005728
CCGTCTT74000.047.15521252
TCACGTG76100.046.7263830
CACGTGA76550.046.7257331
GCCGTCT77150.045.50235451
GTCACGT78050.045.38070329
AACGATC69600.045.25733638
CAGTCAC80100.044.26350427
TGCCGTC81600.043.4066850
TCTCGTA73500.043.3310543
TGAAAAA86800.041.8577164
ACGATCT76700.040.9754339
Produced by FastQC (version 0.11.2)