FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_2500_250_2_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_2500_250_2_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11954036
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTCGTATGC381400.3190554219512138TruSeq Adapter, Index 10 (97% over 36bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC144320.12072909936024954No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA128260.10729430629119738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG58350.055.2999946
CGTATGC61800.052.04340744
TCGTATG62450.051.38942743
TCACTCG66100.050.8286330
CTCGTAT57200.050.23261342
GTATGCC64750.050.104345
GTCACTC69650.048.1891129
AGTCACT69950.047.98283828
ACGTCTG70950.047.80043415
CACGTCT72550.046.93961314
CAGTCAC72400.046.55227327
ACACGTC78150.043.71059813
CACTCGG78650.042.94051731
GTCTGAA80450.042.32742317
ATGCCGT78250.041.5497347
ACTCGGC83700.040.1003532
GGCAATC74700.039.26238336
CACACGT87650.039.13272512
CCAGTCA86850.038.92818526
TCTCGTA74350.038.31617441
Produced by FastQC (version 0.11.2)