Basic Statistics
Measure | Value |
---|---|
Filename | HH2TCBGX7_n01_2500_250_1_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 13081220 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGC | 62549 | 0.4781587650081567 | TruSeq Adapter, Index 9 (97% over 36bp) |
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC | 21130 | 0.16152927632132172 | No Hit |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA | 17733 | 0.13556075044988158 | No Hit |
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC | 16810 | 0.1285048336470146 | No Hit |
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA | 16178 | 0.1236734799965141 | No Hit |
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA | 15240 | 0.11650289498991685 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 7945 | 0.0 | 59.027695 | 46 |
TCGTATG | 8235 | 0.0 | 57.203556 | 43 |
CTCGTAT | 8045 | 0.0 | 57.162457 | 42 |
GTATGCC | 8390 | 0.0 | 56.188686 | 45 |
CGTATGC | 8520 | 0.0 | 55.49544 | 44 |
CTATAAT | 8745 | 0.0 | 55.14963 | 33 |
ACGTCTG | 9015 | 0.0 | 54.04224 | 15 |
AGTCACT | 8910 | 0.0 | 53.85338 | 28 |
CACGTCT | 9090 | 0.0 | 53.635056 | 14 |
TAATATC | 8785 | 0.0 | 53.18523 | 36 |
CACTATA | 9060 | 0.0 | 53.03801 | 31 |
TCACTAT | 9230 | 0.0 | 52.32657 | 30 |
CAGTCAC | 9280 | 0.0 | 51.93249 | 27 |
TATAATA | 9410 | 0.0 | 51.28923 | 34 |
ACACGTC | 9575 | 0.0 | 50.95504 | 13 |
TATCTCG | 9160 | 0.0 | 50.242382 | 39 |
GTCTGAA | 9895 | 0.0 | 49.270298 | 17 |
ATGCCGT | 9610 | 0.0 | 49.056408 | 47 |
ATAATAT | 10330 | 0.0 | 46.75542 | 35 |
CCGTCTT | 10075 | 0.0 | 46.481045 | 50 |