FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_2500_250_1_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_2500_250_1_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13081220
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGC625490.4781587650081567TruSeq Adapter, Index 9 (97% over 36bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC211300.16152927632132172No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA177330.13556075044988158No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC168100.1285048336470146No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA161780.1236734799965141No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA152400.11650289498991685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG79450.059.02769546
TCGTATG82350.057.20355643
CTCGTAT80450.057.16245742
GTATGCC83900.056.18868645
CGTATGC85200.055.4954444
CTATAAT87450.055.1496333
ACGTCTG90150.054.0422415
AGTCACT89100.053.8533828
CACGTCT90900.053.63505614
TAATATC87850.053.1852336
CACTATA90600.053.0380131
TCACTAT92300.052.3265730
CAGTCAC92800.051.9324927
TATAATA94100.051.2892334
ACACGTC95750.050.9550413
TATCTCG91600.050.24238239
GTCTGAA98950.049.27029817
ATGCCGT96100.049.05640847
ATAATAT103300.046.7554235
CCGTCTT100750.046.48104550