FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_2500_125_2_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_2500_125_2_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18794546
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATCCAATCTCGTAT398950.21226902740827044TruSeq Adapter, Index 19 (97% over 37bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC345930.18405871575722021No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA283760.15097997046590006No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA274500.14605300920809686No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA263730.14032262338233656No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC251450.13378881298861914No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA238410.12685062996467167No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTT235720.12541936368135734No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAA223240.11877913943757938No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG220460.11729998692173783No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGA193510.1029607206260795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG54700.058.8651548
AGTCACG56950.057.15404528
CTCGTAT53550.055.29133644
GTCACGT59550.054.59915529
GTATGCC59000.054.5142147
TCGTATG59750.054.1226545
CACGTGA61200.053.1261331
ACGTGAT61800.052.7244532
TCACGTG62300.052.4689930
ACGTCTG64200.051.95318215
CACGTCT65150.051.14175814
CGTATGC63400.051.117346
ACACGTC67000.049.83424413
CAGTCAC65750.049.82378427
GTCTGAA70000.047.59735517
CGTGATC68950.047.00296433
ATGCCGT70600.045.65816549
AGCACAC78400.043.08002510
GCCGTCT74600.042.69437851
CTGAACT79950.041.80505419
Produced by FastQC (version 0.11.2)