Basic Statistics
Measure | Value |
---|---|
Filename | HH2TCBGX7_n01_2500_125_1_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 15596891 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGC | 31514 | 0.2020530886572202 | TruSeq Adapter, Index 9 (97% over 36bp) |
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC | 23829 | 0.15278044835986865 | No Hit |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA | 20553 | 0.13177626233330733 | No Hit |
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC | 18326 | 0.11749777567849899 | No Hit |
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA | 17462 | 0.11195821013303228 | No Hit |
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA | 16996 | 0.10897043519763008 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 5100 | 0.0 | 53.52616 | 46 |
TCGTATG | 5215 | 0.0 | 52.412254 | 43 |
CTCGTAT | 5085 | 0.0 | 51.96275 | 42 |
GTATGCC | 5295 | 0.0 | 51.951183 | 45 |
CGTATGC | 5315 | 0.0 | 51.623672 | 44 |
ACGTCTG | 6035 | 0.0 | 48.657528 | 15 |
CACGTCT | 6195 | 0.0 | 47.62682 | 14 |
CAGTCAC | 6235 | 0.0 | 46.478218 | 27 |
AGTCACC | 6275 | 0.0 | 46.014473 | 28 |
ACACGTC | 6425 | 0.0 | 45.867565 | 13 |
CATTTTA | 6360 | 0.0 | 45.564877 | 34 |
TTATCTC | 5955 | 0.0 | 44.431835 | 38 |
GTCTGAA | 6785 | 0.0 | 43.17364 | 17 |
ATGCCGT | 6460 | 0.0 | 42.095238 | 47 |
ACCATTT | 7270 | 0.0 | 40.05387 | 32 |
AGCACAC | 7615 | 0.0 | 39.25199 | 10 |
ATTTTAT | 7595 | 0.0 | 38.15583 | 35 |
CTGAACT | 7895 | 0.0 | 37.635582 | 19 |
CCGTCTT | 7225 | 0.0 | 37.542297 | 50 |
GCCGTCT | 7250 | 0.0 | 37.02664 | 49 |