FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_2500_125_1_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_2500_125_1_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15596891
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGC315140.2020530886572202TruSeq Adapter, Index 9 (97% over 36bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC238290.15278044835986865No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA205530.13177626233330733No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC183260.11749777567849899No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA174620.11195821013303228No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA169960.10897043519763008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG51000.053.5261646
TCGTATG52150.052.41225443
CTCGTAT50850.051.9627542
GTATGCC52950.051.95118345
CGTATGC53150.051.62367244
ACGTCTG60350.048.65752815
CACGTCT61950.047.6268214
CAGTCAC62350.046.47821827
AGTCACC62750.046.01447328
ACACGTC64250.045.86756513
CATTTTA63600.045.56487734
TTATCTC59550.044.43183538
GTCTGAA67850.043.1736417
ATGCCGT64600.042.09523847
ACCATTT72700.040.0538732
AGCACAC76150.039.2519910
ATTTTAT75950.038.1558335
CTGAACT78950.037.63558219
CCGTCTT72250.037.54229750
GCCGTCT72500.037.0266449