FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_2500_062_2_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_2500_062_2_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8957179
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC141270.15771706694708232TruSeq Adapter, Index 7 (97% over 35bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC123630.1380233665085849No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC101320.11311597099935147No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA93170.10401712414142891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG21750.050.68752346
TCGTATG22000.050.1104143
CTCGTAT22300.047.55299442
ACGATAT23150.047.31982435
GTATGCC23650.047.05906745
CGTATGC24100.045.88916444
CGATATC24000.043.60233336
CACGTCT26450.043.40179414
ACGTCTG27500.041.744415
AGTCACC27350.041.5901428
CAGTCAC28650.039.4586527
ACACGTC29200.039.43437613
CCACGAT29350.038.8740133
ATGCCGT30100.036.6269847
GTCTGAA32150.036.032117
AGCACAC35400.033.31966810
CACGATA35100.032.6054734
GCCGTCT34250.031.78110549
ACCACGA37300.030.96419132
CTGAACT38300.030.42919319
Produced by FastQC (version 0.11.2)