FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_2500_062_1_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_2500_062_1_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10875829
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC168410.15484796607228746No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGC161930.14888979957297968TruSeq Adapter, Index 1 (97% over 37bp)
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC138710.12753970295046013No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA128770.11840016977096642No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA121710.11190871059116506No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA120140.11046514247327721No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA111220.1022634688353412No Hit
TCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAG110380.10149111391876425No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG110110.10124285698129311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25850.054.9685746
CTCGTAT24050.052.9706542
TCGTATG25950.050.98018643
GTATGCC28550.050.75060345
GTCACAT35350.048.6132929
AGTCACA35400.048.44598828
CGTATGC30050.047.86789744
ACGTCTG36400.047.5953815
CAGTCAC36450.046.76235627
CACGTCT36950.046.69726614
ACACGTC38750.044.88938513
GTCTGAA39300.044.2597417
ATGCCGT33850.040.21943347
CTGAACT44200.039.7488419
CACATGA43850.039.5077831
AGCACAC44500.039.167910
TGAACTC45600.037.991420
ACATGAG46300.037.49398832
TGAGCAT45950.035.64653435
CATCTCG37950.035.22853539