FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_2500_031_2_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_2500_031_2_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9914319
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT252760.2549443890195585TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAA36650.057.9646736
ACGTAAT38350.055.12140337
TATGCCG40100.052.9792948
CGTATGC41800.050.73936546
CTCGTAT38550.050.56741744
TCGTATG42850.049.3326945
CGTAATC40000.048.99781438
GTATGCC43400.048.78863547
GTACGTA44400.048.4773235
ACGTCTG47150.046.39358515
CACGTCT47600.046.10204714
CAGTCAC47400.045.26306527
CCGTACG48100.044.3849433
CGTACGT48700.043.98138834
AGTCACC49800.043.15177528
ACACGTC52100.042.5231713
TAATCTC46650.042.08709340
GTCTGAA54600.040.7665917
ACCGTAC53700.040.60381732
ATGCCGT55400.038.41100349
Produced by FastQC (version 0.11.2)