FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_2500_031_1_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_2500_031_1_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9936717
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG424580.4272839812183441No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG174360.17547042951912586No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC138900.13978459887707378No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC117460.11820805604104453No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT116880.11762436225163703TruSeq Adapter, Index 18 (97% over 40bp)
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA110570.11127417637032432No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA104100.10476297151262334No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA101430.10207596734414395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG22000.040.8855848
TCGTATG24150.038.11387345
CTCGTAT23500.036.63612444
CGTATGC25050.036.4652746
GTATGCC25050.036.3259247
CGCACAT32200.028.1512137
ATGCCGT33250.027.68381949
TGGGGGG48850.027.3764131
AGTCACG34250.026.77361728
CCGCACA34450.026.10941136
ACGTCCG36000.025.37438832
CCGTCTT38050.023.823752
GTCACGT38850.023.7835729
GTCCGCA40800.023.0751934
GCCGTCT40450.022.23724251
CAGTCAC43100.021.6008427
GCCAGTA34500.021.512681
TCACGTC43550.021.29630930
CATGCGT21200.020.9682675
TCTCGTA40800.020.9301343