FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_1250_250_2_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_1250_250_2_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11682810
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC184400.15783873913895716No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA170410.1458638803507033No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA145420.12447347855524483No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA144350.1235576030081804No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC128310.10982802938676568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG60950.057.9962346
TCGTATG62800.056.17556443
CGTATGC62750.056.0530144
CTCGTAT62000.054.86778642
TCACTCG65450.054.75618430
GTATGCC64850.054.4004745
AGTCACT67450.053.39338328
ACGTCTG70450.051.5173615
GTCACTC70000.050.9981329
CAGTCAC70450.050.97132527
CACGTCT72700.050.06801214
ACACGTC75200.048.58989713
ATGCCGT73250.048.40116547
GTCTGAA75950.047.87843317
GCCGTCT78400.045.0004749
CCGTCTT78300.044.78993250
AGCACAC87300.042.53712510
TCTCGTA79250.042.39501641
CACACGT87750.041.6802812
CCAGTCA86750.041.51539626
Produced by FastQC (version 0.11.2)