FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_1250_250_1_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_1250_250_1_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15482835
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACAGCATCTCGTATGC291800.1884667762719166TruSeq Adapter, Index 7 (97% over 35bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC238330.1539317573299722No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA201760.13031205202406407No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA187670.12121165148372376No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA174840.11292505539198731No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC163540.10562665041641275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG49850.052.4451246
AGTCACT82850.051.70739428
ACGTCTG87100.049.58474715
CAGTCAC88600.048.39147627
CTCGTAT49800.047.8583442
CACGTCT90800.047.67985514
TCGTATG48400.047.00123643
CACTACA92100.046.73993731
CGTATGC54900.046.47239344
ACACGTC93950.046.2303813
GTATGCC59350.045.8194645
ACTACAG94550.045.49281732
GTCTGAA95200.045.2914717
CACACGT105700.041.09124412
AGCACAC110150.039.87691510
CCAGTCA108850.039.485926
CTGAACT113450.038.00583619
TCTGAAC115250.037.5944518
ACAGCAT113550.036.33951635
ATGCCGT68600.036.32523347
Produced by FastQC (version 0.11.2)