FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_1250_125_2_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_1250_125_2_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12680201
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC324800.25614735917829695TruSeq Adapter, Index 3 (97% over 37bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC166150.1310310459589718No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA141700.11174901722772375No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA134770.10628380417629026No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA129860.10241162580940161No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC128930.10167819894968542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG47700.054.5878546
CGTATGC50350.051.99242844
CTCGTAT48450.051.35907742
TCGTATG51650.050.68381543
GTATGCC52200.049.68086645
ACGTCTG54900.048.96164315
CACGTCT57300.046.7887314
CAGTCAC57550.045.3070827
AGTCACC58650.044.3976628
ACACGTC61650.043.60122713
TCACCTA60000.043.5141730
CACCTAG61650.042.2927931
CTATCTC57700.042.15695638
GCTATCT58250.041.75907537
ATGCCGT63900.040.6945547
TATCTCG60350.040.18808739
GTCTGAA67200.039.99723417
ACCTAGC68750.038.0793232
CCTAGCT71750.036.8283233
CCGTCTT71650.035.8546350