Basic Statistics
Measure | Value |
---|---|
Filename | HH2TCBGX7_n01_1250_125_2_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 12680201 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC | 32480 | 0.25614735917829695 | TruSeq Adapter, Index 3 (97% over 37bp) |
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC | 16615 | 0.1310310459589718 | No Hit |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA | 14170 | 0.11174901722772375 | No Hit |
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA | 13477 | 0.10628380417629026 | No Hit |
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA | 12986 | 0.10241162580940161 | No Hit |
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC | 12893 | 0.10167819894968542 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 4770 | 0.0 | 54.58785 | 46 |
CGTATGC | 5035 | 0.0 | 51.992428 | 44 |
CTCGTAT | 4845 | 0.0 | 51.359077 | 42 |
TCGTATG | 5165 | 0.0 | 50.683815 | 43 |
GTATGCC | 5220 | 0.0 | 49.680866 | 45 |
ACGTCTG | 5490 | 0.0 | 48.961643 | 15 |
CACGTCT | 5730 | 0.0 | 46.78873 | 14 |
CAGTCAC | 5755 | 0.0 | 45.30708 | 27 |
AGTCACC | 5865 | 0.0 | 44.39766 | 28 |
ACACGTC | 6165 | 0.0 | 43.601227 | 13 |
TCACCTA | 6000 | 0.0 | 43.51417 | 30 |
CACCTAG | 6165 | 0.0 | 42.29279 | 31 |
CTATCTC | 5770 | 0.0 | 42.156956 | 38 |
GCTATCT | 5825 | 0.0 | 41.759075 | 37 |
ATGCCGT | 6390 | 0.0 | 40.69455 | 47 |
TATCTCG | 6035 | 0.0 | 40.188087 | 39 |
GTCTGAA | 6720 | 0.0 | 39.997234 | 17 |
ACCTAGC | 6875 | 0.0 | 38.07932 | 32 |
CCTAGCT | 7175 | 0.0 | 36.82832 | 33 |
CCGTCTT | 7165 | 0.0 | 35.85463 | 50 |