FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_1250_125_1_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_1250_125_1_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13384730
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC152770.11413752836254448No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA133880.10002443082527626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG48550.041.09110615
CACGTCT50000.040.17946214
CAGTCAC53550.036.66631327
AGTCACC54650.036.24864228
ACACGTC56600.035.9889213
GTCTGAA61150.032.85099417
CACACGT66100.031.08128212
TATGCCG20350.030.2689646
AGCACAC70650.029.4771110
CCAGTCA68650.028.65252326
CTGAACT73150.027.7489619
ATCGGAA74250.027.2938922
CGTATGC21600.027.0586144
TCTGAAC78900.025.8154118
TGAACTC80650.025.12525620
GTATGCC24850.024.928545
CTCCAGT81550.024.59176424
TCCAGTC81650.024.56210325
CATGGCA81550.024.41963634
GAGCACA86750.024.2078939
Produced by FastQC (version 0.11.2)