FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_1250_062_2_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_1250_062_2_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11052872
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC250890.22699077669586692TruSeq Adapter, Index 2 (97% over 36bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC162660.14716537023137516No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC134150.12137116941189584No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA125200.11327372650293968No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA121750.1101523658285376No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA113270.10248015176507971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33400.056.8985646
TCGTATG34650.055.14895643
CTCGTAT32700.053.94293642
GTATGCC36150.053.1510645
CGTATGC38200.050.3903344
CAGTCAC40050.048.50093527
AGTCACC39850.048.21760628
ACGTCTG41700.047.7566615
CACGTCT41650.047.72995814
CTAATCT36250.047.3092237
ATGCCGT40700.046.69337547
TAATCTC37200.046.2894338
ACACGTC44000.045.34044613
GTCTGAA45450.043.73814817
GCCGTCT44300.042.58382849
CCGTCTT46450.040.8388350
AGCACAC50400.039.6527310
TGCCGTC48350.039.37858648
CTGAACT51400.038.6071619
TGAACTC56000.035.6860120
Produced by FastQC (version 0.11.2)