Basic Statistics
Measure | Value |
---|---|
Filename | HH2TCBGX7_n01_1250_062_1_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 11019689 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT | 23092 | 0.20955219335137315 | TruSeq Adapter, Index 25 (97% over 44bp) |
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC | 16298 | 0.14789891075873374 | No Hit |
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC | 13613 | 0.12353343184186051 | No Hit |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA | 12792 | 0.11608313083971789 | No Hit |
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA | 11561 | 0.1049122166696356 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 3305 | 0.0 | 55.06672 | 48 |
TCGTATG | 3460 | 0.0 | 53.406906 | 45 |
CTCGTAT | 3355 | 0.0 | 52.886303 | 44 |
GTATGCC | 3525 | 0.0 | 51.925907 | 47 |
CGTATGC | 3910 | 0.0 | 46.633774 | 46 |
AGTCACA | 4080 | 0.0 | 45.89431 | 28 |
ACGTCTG | 4095 | 0.0 | 45.81125 | 15 |
CACGTCT | 4175 | 0.0 | 45.351967 | 14 |
CAGTCAC | 4135 | 0.0 | 45.19943 | 27 |
TGATATA | 4160 | 0.0 | 45.09361 | 36 |
TATATCT | 3995 | 0.0 | 44.852314 | 39 |
ATGCCGT | 4130 | 0.0 | 43.814266 | 49 |
ACTGATA | 4335 | 0.0 | 43.757027 | 34 |
GTCACAC | 4345 | 0.0 | 43.175194 | 29 |
ACACGTC | 4435 | 0.0 | 42.772144 | 13 |
GTCTGAA | 4570 | 0.0 | 40.97124 | 17 |
GCCGTCT | 4465 | 0.0 | 40.762695 | 51 |
GATATAT | 4645 | 0.0 | 40.61127 | 37 |
CTGATAT | 4760 | 0.0 | 39.335476 | 35 |
ATATATC | 4610 | 0.0 | 39.097576 | 38 |