FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_1250_062_1_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_1250_062_1_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11019689
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT230920.20955219335137315TruSeq Adapter, Index 25 (97% over 44bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC162980.14789891075873374No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC136130.12353343184186051No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA127920.11608313083971789No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA115610.1049122166696356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG33050.055.0667248
TCGTATG34600.053.40690645
CTCGTAT33550.052.88630344
GTATGCC35250.051.92590747
CGTATGC39100.046.63377446
AGTCACA40800.045.8943128
ACGTCTG40950.045.8112515
CACGTCT41750.045.35196714
CAGTCAC41350.045.1994327
TGATATA41600.045.0936136
TATATCT39950.044.85231439
ATGCCGT41300.043.81426649
ACTGATA43350.043.75702734
GTCACAC43450.043.17519429
ACACGTC44350.042.77214413
GTCTGAA45700.040.9712417
GCCGTCT44650.040.76269551
GATATAT46450.040.6112737
CTGATAT47600.039.33547635
ATATATC46100.039.09757638