FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_1250_031_2_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_1250_031_2_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8039102
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT1140531.4187281116721744TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG171550.066.3865528
CGTTTCG171500.066.26305433
TATGCCG139400.065.4792248
ACGTTTC174300.065.2989832
TTTCGGA174250.065.1972135
TTCGGAA172650.065.07204436
CTCGTAT131000.064.86680644
CACGTTT176000.064.80624431
ACGTCTG180900.064.3885715
GTATGCC143550.064.3408447
CGTATGC142050.064.3303946
GTCACGT177550.064.1234129
CACGTCT182250.064.1036714
TCGTATG140250.063.907945
TCGGAAT173050.063.82948737
CAGTCAC179900.063.4029127
ACACGTC185950.062.77245713
GTCTGAA188150.061.86797317
CCAGTCA187900.060.74110426
GTTTCGG188700.060.39010234
Produced by FastQC (version 0.11.2)