FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_1250_031_1_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_1250_031_1_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11089942
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC141960.12800788317919065No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC123310.11119084301793464TruSeq Adapter, Index 3 (97% over 37bp)
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC117520.10596989596519081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG24450.040.50883546
CTCGTAT25650.038.34010742
TCGTATG26800.037.60902443
CGTATGC26850.037.40864644
AGTCACG27250.036.86152328
GTATGCC29050.034.09420445
ACGTAGA31200.032.19403532
CACGTAG32650.030.97839231
GTCACGT33450.030.23845529
CGTAGAG33700.029.70189933
CAGTCAC34500.029.11526127
TCACGTA36850.027.82750730
ACGTCTG36700.027.75139215
CACGTCT38100.027.46642514
TAGAGAT36600.026.77460735
GTAGAGA39850.025.03021834
ATGCCGT40650.024.79568747
ACACGTC42450.024.23971613
GTCTGAA44650.022.96605517
TCTCGTA44850.021.7708941
Produced by FastQC (version 0.11.2)