FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_0625_250_2_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_0625_250_2_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11091594
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGAATCTCGTATGC319920.28843464699483234TruSeq Adapter, Index 6 (97% over 36bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC191540.17268933572577577No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA160670.14485744790153696No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA135200.12189411188328746No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC130220.117404225217764No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA128680.11601578636938929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAT71200.058.79027635
AGTCACT79450.054.31619628
ACGTCTG80750.054.2221715
TATGCCG52900.053.589546
CGTATGC51800.053.24083744
ACTCCCG81000.052.7575932
CTCGTAT50800.052.15315642
GTCACTC82800.051.78046829
GTATGCC56400.051.6291145
CACGTCT85050.051.60423714
TCGTATG51300.051.0308343
CAGTCAC84100.051.0216727
CGAATCT65400.050.89334537
ACACGTC87300.050.1140913
CACTCCC85550.049.74621631
GTCTGAA90450.048.36708517
CACACGT94150.046.61646712
TCACTCC92200.046.5004930
CCAGTCA95100.045.08350826
AGCACAC98950.044.63910710
Produced by FastQC (version 0.11.2)