FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_0625_250_1_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_0625_250_1_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15266605
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC596940.3910103130329239TruSeq Adapter, Index 3 (97% over 36bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC204600.13401800858802596No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA176300.11548081580678873No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA168030.11006376335799609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG88450.056.3854246
TCGTATG91500.054.96364643
CGTATGC92200.054.7361444
CTCGTAT85750.054.3233642
GTATGCC97550.051.34074445
AGTCACT99150.051.1839328
ACGTCTG101000.050.5247815
CACGTCT105450.048.42566314
CAGTCAC105600.047.9911927
CTAATCG107200.047.3397433
ACACGTC109100.046.8376413
AATCGAT105000.045.3649135
ATGCCGT109850.045.27391447
GTCTGAA113800.044.8396517
CACACGT117150.043.8281612
CACTAAT121250.042.77716431
ACTAATC119450.042.51432832
TCTCGTA105950.042.47982441
CCGTCTT118400.042.24297350
CCAGTCA122050.041.60906626
Produced by FastQC (version 0.11.2)