FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_0625_125_2_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_0625_125_2_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15093124
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTCGTATGC250060.16567809288521052TruSeq Adapter, Index 7 (97% over 37bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC231860.15361962175623814No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA199740.13233840787367812No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA189300.12542135080848735No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA182940.12120751144693437No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC180970.11990228133022694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG39750.049.65691446
TCGTATG40650.048.72936643
CTCGTAT39800.048.2753742
CGTATGC42250.046.46982644
GTATGCC44500.045.3788645
ACGTCTG48800.045.25584415
CACGTCT48500.045.24741714
CAGTCAC50850.042.7427927
AGTCACC51900.042.1476628
ACACGTC52500.041.8666613
GTCTGAA56900.038.68786217
GAATATC55300.037.59319336
ATGCCGT54750.036.0525247
AGCACAC66600.033.73929610
AATATCT60700.033.21086537
GCCAGTA50850.033.184691
TATCTCG59650.032.7384939
CTGAACT69650.032.30952519
CCGTCTT61000.031.61433250
GCCGTCT62200.030.6666949
Produced by FastQC (version 0.11.2)