FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_0625_125_1_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_0625_125_1_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18805525
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC417040.2217646143885906TruSeq Adapter, Index 7 (97% over 36bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC295880.15733674013355117No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA283850.1509396839492649No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA248610.13220051022239473No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC236270.1256386088662773No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA223070.11861939509798317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG71400.054.26134546
CTCGTAT62850.052.84533742
TCGTATG73100.052.7124743
CGTATGC74600.052.16848844
GTATGCC75500.051.45376245
ACGTCTG85650.047.6874715
CACGTCT87050.047.08134514
CAGTCAC87050.046.39673227
AGTCACC88900.045.2738728
ATGCCGT85950.045.19813547
ACACGTC92350.044.45512813
GTCTGAA96250.042.50645417
CCGTCTT99300.038.91142750
GCCGTCT98950.038.5537749
AGCACAC109400.037.78363410
CTGAACT111850.036.67203519
TGCCGTC105150.036.61316348
CACACGT114550.035.80908612
CCAGTCA113700.035.5530226
GAGCACA122750.034.244559
Produced by FastQC (version 0.11.2)