FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_0625_062_2_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_0625_062_2_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8691846
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC292320.33631520852992564TruSeq Adapter, Index 7 (97% over 37bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC144910.16671947478130653No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC118910.13680638152125565No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA115430.13280262903875656No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA107360.12351806509227155No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA106510.12254013704338525No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA90580.10421261490366948No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG87570.10074959910702515No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGA86980.10007080199073938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41100.060.3721746
TCGTATG42500.058.13706643
GTATGCC42900.057.59466645
CTCGTAT40650.057.5113142
CGTATGC44300.055.93284244
AGTCACC46600.053.62436328
CAGTCAC46600.053.2491527
CACGTCT47600.052.7922114
ACGTCTG47600.052.7186815
ATGCCGT46900.052.68314747
ACACGTC48750.051.47535713
GTCTGAA49500.050.69280617
GCCGTCT50250.048.82494449
AGCACAC54250.046.9684710
CCGTCTT52950.046.46775450
TGCCGTC54450.045.50773648
AGATCTC50250.045.06252738
AAAGATC51150.044.81676536
CTGAACT56900.044.4691119
TGAACTC57800.043.83798620