Basic Statistics
Measure | Value |
---|---|
Filename | HH2TCBGX7_n01_0625_062_2_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8691846 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC | 29232 | 0.33631520852992564 | TruSeq Adapter, Index 7 (97% over 37bp) |
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC | 14491 | 0.16671947478130653 | No Hit |
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC | 11891 | 0.13680638152125565 | No Hit |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA | 11543 | 0.13280262903875656 | No Hit |
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATA | 10736 | 0.12351806509227155 | No Hit |
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA | 10651 | 0.12254013704338525 | No Hit |
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA | 9058 | 0.10421261490366948 | No Hit |
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG | 8757 | 0.10074959910702515 | No Hit |
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGA | 8698 | 0.10007080199073938 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 4110 | 0.0 | 60.37217 | 46 |
TCGTATG | 4250 | 0.0 | 58.137066 | 43 |
GTATGCC | 4290 | 0.0 | 57.594666 | 45 |
CTCGTAT | 4065 | 0.0 | 57.51131 | 42 |
CGTATGC | 4430 | 0.0 | 55.932842 | 44 |
AGTCACC | 4660 | 0.0 | 53.624363 | 28 |
CAGTCAC | 4660 | 0.0 | 53.24915 | 27 |
CACGTCT | 4760 | 0.0 | 52.79221 | 14 |
ACGTCTG | 4760 | 0.0 | 52.71868 | 15 |
ATGCCGT | 4690 | 0.0 | 52.683147 | 47 |
ACACGTC | 4875 | 0.0 | 51.475357 | 13 |
GTCTGAA | 4950 | 0.0 | 50.692806 | 17 |
GCCGTCT | 5025 | 0.0 | 48.824944 | 49 |
AGCACAC | 5425 | 0.0 | 46.96847 | 10 |
CCGTCTT | 5295 | 0.0 | 46.467754 | 50 |
TGCCGTC | 5445 | 0.0 | 45.507736 | 48 |
AGATCTC | 5025 | 0.0 | 45.062527 | 38 |
AAAGATC | 5115 | 0.0 | 44.816765 | 36 |
CTGAACT | 5690 | 0.0 | 44.46911 | 19 |
TGAACTC | 5780 | 0.0 | 43.837986 | 20 |