FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_0625_062_1_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_0625_062_1_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9614990
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC164760.17135743250903018TruSeq Adapter, Index 22 (97% over 37bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC152900.15902252628447872No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC124840.1298389285896293No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTA112840.11735841638940861No Hit
CCCGACTGTCCCTATTAATCATTACTCCGATCCCGAAGGCCAACACAATA108290.11262622218015828No Hit
TCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAG100770.1048051012013533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG24050.055.44629328
TATGCCG23400.054.4417846
TCGTATG24500.052.56885543
CTCGTAT23350.050.81147842
GTATGCC25100.050.3361845
CGTATGC26250.048.53095644
CAGTCAC27750.048.0539527
ACGTCTG29250.047.26436215
CACGTCT29450.046.5866114
GTCACGG29900.044.598129
ATGCCGT29750.043.41009547
ACACGTC32200.043.04271313
GTCTGAA32200.042.8239917
TAGCATC28450.041.0882636
GGTAGCA33250.039.89340634
GCCGTCT33700.037.38786749
AGCACAC36700.037.28855510
CTGAACT37300.036.7812319
CCGTCTT35650.035.14645850
TGCCGTC36700.034.80864748
Produced by FastQC (version 0.11.2)