FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_0625_031_2_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_0625_031_2_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8220739
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC102360.12451435327164626No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC86760.10553795711066852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17950.037.4367748
AGTCACG18700.035.9346428
CGTATGC19300.034.9979946
CTCGTAT18300.034.8061144
TCGTATG19100.033.89857545
GCCTTAT19850.033.14711437
TCACGTG20900.032.6530330
GTATGCC20600.032.61948847
GTCACGT22300.030.44742229
ACGTCTG23600.029.21584715
TTATCTC22400.028.7476540
CACGTCT24550.028.5131614
GTACTCT23050.025.6624723
CAGTCAC26400.025.58627327
CTTATCT25400.025.07653839
ACACGTC28400.024.64782113
GACTATC19400.024.35649355
CCTTATC27200.023.9327438
GTCTGAA29350.023.84886217
ATGCCGT28850.023.77809749
Produced by FastQC (version 0.11.2)