FastQCFastQC Report
Sat 25 Aug 2018
HH2TCBGX7_n01_0625_031_1_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2TCBGX7_n01_0625_031_1_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10196496
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC107070.10500666111181725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18650.034.15376346
TCGTATG19950.030.70019743
GTATGCC20700.030.26416645
CTCGTAT19100.030.05097842
CGTATGC21550.029.39526244
ACGTCTG26100.026.68512315
TCTAGTT38750.026.1021587
GTACTCT36800.026.0616533
TTACCTC36300.025.93595915
CACGTCT27350.025.84941314
CAGTCAC27000.024.62910527
TCTAATG37750.024.56865120
AGTCACC27650.024.3032828
GACTATC33350.023.92811455
TGTTACC38450.023.75773813
CTAATGC38950.023.72186721
ACCTACT35150.023.39921833
TAATGCT39400.023.18444422
ACACGTC30400.023.02593813
TACTCTA42250.022.451434
Produced by FastQC (version 0.11.2)