FastQCFastQC Report
Mon 24 Sep 2018
HH2MLBGX7_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2MLBGX7_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32613213
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21511846.596050502598441No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT661940.2029668159343883No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCCATATAAGAAAA380680.11672569642248987No Hit
GCATAGCTCTTAAACCATATAGGTCCTTGGGAACCCCCCATATAAGAAAC358220.1098389171284657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGC629050.043.1855741
TGTCCGA301700.043.0854846
CATAGCT630900.042.6902122
TTGTCCG301950.042.6628275
ATAGCTC633900.042.618843
CCGATTC298350.042.496249
TAGCTCT628150.042.427244
TCCGATT304800.041.8698778
GATTCCC306550.041.70161411
AGTTGTC315400.041.6468433
CGATTCC306800.041.29325510
GGGAACC204800.040.60336329
TAGGTCC220950.040.45084420
GTTGTCC318350.040.4348564
TGTACCC208450.040.42594510
GCAGTTG331250.040.2254181
ATATAGG233900.040.02438417
TGGGAAC209800.039.98076628
CATATAG233900.039.86540616
GGAACCC209250.039.50985730